Journal:

Article Title: In Vitro Activity of TD-6424 against Staphylococcus aureus

doi: 10.1128/AAC.47.11.3602-3604.2003

Figure Lengend Snippet: In vitro activities of TD-6424 and comparator antibiotics against MRSA and MSSA

Article Snippet: Linezolid at 8 μg/ml reduced the initial inoculum from log 10 5.76 ± 0.07 CFU/ml to log 10 2.73 ± 0.08 CFU/ml by 4 h, and vancomycin at 32 μg/ml decreased it from log 10 5.76 ± 0.07 CFU/ml to log 10 4.82 ± 0.02 CFU/ml over this period. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Time-kill curves for MSSA (ATCC 13709) (A), MRSA (ATCC 33591) (B), and GISA (HIP 5836) (C). (A) Vancomycin at 4 times the MIC (⧫), nafcillin at 4 times the MIC (▪), linezolid at 4 times the MIC (▴), and TD-6424 at 4 times the MIC (▵); (B) vancomycin at 8 times the MIC (⧫), linezolid at 8 times the MIC (▴), and TD-6424 at 8 (▵) and 32 (▪) times the MIC; (C) linezolid at 4 times the MIC (▴), vancomycin at 4 times the MIC (⧫), and TD-6424 at 4 (▵), 8 (▪), and 16 (□) times the MIC.

Techniques: In Vitro

Journal:

Article Title: In Vitro Activity of TD-6424 against Staphylococcus aureus

doi: 10.1128/AAC.47.11.3602-3604.2003

Figure Lengend Snippet: Time-kill curves for MSSA (ATCC 13709) (A), MRSA (ATCC 33591) (B), and GISA (HIP 5836) (C). (A) Vancomycin at 4 times the MIC (⧫), nafcillin at 4 times the MIC (▪), linezolid at 4 times the MIC (▴), and TD-6424 at 4 times the MIC (▵); (B) vancomycin at 8 times the MIC (⧫), linezolid at 8 times the MIC (▴), and TD-6424 at 8 (▵) and 32 (▪) times the MIC; (C) linezolid at 4 times the MIC (▴), vancomycin at 4 times the MIC (⧫), and TD-6424 at 4 (▵), 8 (▪), and 16 (□) times the MIC. ✻, bacterial growth control. The nafcillin MIC was 1 μg/ml for ATCC 13709. Vancomycin MICs were 1, 1, and 8 μg/ml for ATCC 13709, ATCC 33591, and HIP 5836, respectively; the corresponding values were 2, 2, and 2 μg/ml for linezolid and 1, 1, and 2 μg/ml for TD-6424.

Article Snippet: Linezolid at 8 μg/ml reduced the initial inoculum from log 10 5.76 ± 0.07 CFU/ml to log 10 2.73 ± 0.08 CFU/ml by 4 h, and vancomycin at 32 μg/ml decreased it from log 10 5.76 ± 0.07 CFU/ml to log 10 4.82 ± 0.02 CFU/ml over this period. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Time-kill curves for MSSA (ATCC 13709) (A), MRSA (ATCC 33591) (B), and GISA (HIP 5836) (C). (A) Vancomycin at 4 times the MIC (⧫), nafcillin at 4 times the MIC (▪), linezolid at 4 times the MIC (▴), and TD-6424 at 4 times the MIC (▵); (B) vancomycin at 8 times the MIC (⧫), linezolid at 8 times the MIC (▴), and TD-6424 at 8 (▵) and 32 (▪) times the MIC; (C) linezolid at 4 times the MIC (▴), vancomycin at 4 times the MIC (⧫), and TD-6424 at 4 (▵), 8 (▪), and 16 (□) times the MIC.

Techniques: Control

Journal:

Article Title: In Vitro Activity of TD-6424 against Staphylococcus aureus

doi: 10.1128/AAC.47.11.3602-3604.2003

Figure Lengend Snippet: PAE for TD-6424 and comparator antibiotics

Article Snippet: Linezolid at 8 μg/ml reduced the initial inoculum from log 10 5.76 ± 0.07 CFU/ml to log 10 2.73 ± 0.08 CFU/ml by 4 h, and vancomycin at 32 μg/ml decreased it from log 10 5.76 ± 0.07 CFU/ml to log 10 4.82 ± 0.02 CFU/ml over this period. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Time-kill curves for MSSA (ATCC 13709) (A), MRSA (ATCC 33591) (B), and GISA (HIP 5836) (C). (A) Vancomycin at 4 times the MIC (⧫), nafcillin at 4 times the MIC (▪), linezolid at 4 times the MIC (▴), and TD-6424 at 4 times the MIC (▵); (B) vancomycin at 8 times the MIC (⧫), linezolid at 8 times the MIC (▴), and TD-6424 at 8 (▵) and 32 (▪) times the MIC; (C) linezolid at 4 times the MIC (▴), vancomycin at 4 times the MIC (⧫), and TD-6424 at 4 (▵), 8 (▪), and 16 (□) times the MIC.

Techniques:

Journal: Indian Journal of Microbiology

Article Title: Melioration in Anti-staphylococcal Activity of Conventional Antibiotic(s) by Organic Acids Present in the Cell Free Supernatant of Lactobacillus paraplantarum

doi: 10.1007/s12088-017-0659-z

Figure Lengend Snippet: Antibiogram of different strains of S. aureus using methicillin and combi 513 octodisc

Article Snippet: However, sensitivity of MRSA to other classes of antibiotics such as co-trimoxazole, gentamicin and ciprofloxacin may be due to the inhibition or interference with folate synthesis, protein synthesis (30S) and DNA gyrase, respectively [ 14 – 16 ]. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 ATCC 9144 MSSA S1 MRSA R1 MRSA R2 MRSA R3 MRSA R4 MRSA R5 MRSA R6 MRSA R7 MRSA R8 MRSA R9 MRSA R10 L.paraplantarum COT S S S S I S I I S I S S I GEN S S S S S S S S S S S S S CTR S I R I R I I I I I I S S CIP S S S S S S I S S S S I I CXM S I R S S S S I R S S S R AMP S R R R R R R R R R R R R AK I S S S I S S I S S S S S CEP S S S S S S S S S S S S S MET S S R R R R R R R R R R R Open in a separate window Cut-off values of different antibiotics according to CLSI guidelines: COT (25 μg): S ≥ 16, I 11–15, R ≤ 10; GEN (10 μg): S ≥ 15, I 13–14, R < 12; CTR (30 μg): S ≥ 21, I 14–20, R ≤ 13; CIP (5 μg): S ≥ 21, I 16–20, R ≤ 15; CXM (30 μg): S ≥ 18, I 15–17, R ≤ 14; AMP (10 μg): S ≥ 29, R ≤ 28; AK (30 μg): S ≥ 17, I 15–16, R ≤ 14; CEP (30 μg): S ≥ 18, I 15–17, R ≤ 14; MET (5 μg): S ≥ 14, I 10–13, R ≤ 9 COT co-trimoxazole, GEN gentamicin, CTR ceftriaxone, CIP ciprofloxacin, CXM cefuroxime, AMP ampicillin, AK amikacin, CEP cephalothin, MET methicillin, S sensitive, I intermediate, R resistant Antibiogram of different strains of S. aureus using methicillin and combi 513 octodisc On the other hand, CFS of all the four Lactobacillus strains ( L. acidophilus , L. casei , L. plantarum , L. paraplantarum ) revealed the anti-staphylococcal activity for the standard, methicillin sensitive and methicillin resistant strains (Table ).

Techniques:

Journal: Indian Journal of Microbiology

Article Title: Melioration in Anti-staphylococcal Activity of Conventional Antibiotic(s) by Organic Acids Present in the Cell Free Supernatant of Lactobacillus paraplantarum

doi: 10.1007/s12088-017-0659-z

Figure Lengend Snippet: Zones of inhibition of CFS (from different strains of Lactobacillus ) against different strains of S. aureus

Article Snippet: However, sensitivity of MRSA to other classes of antibiotics such as co-trimoxazole, gentamicin and ciprofloxacin may be due to the inhibition or interference with folate synthesis, protein synthesis (30S) and DNA gyrase, respectively [ 14 – 16 ]. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 ATCC 9144 MSSA S1 MRSA R1 MRSA R2 MRSA R3 MRSA R4 MRSA R5 MRSA R6 MRSA R7 MRSA R8 MRSA R9 MRSA R10 L.paraplantarum COT S S S S I S I I S I S S I GEN S S S S S S S S S S S S S CTR S I R I R I I I I I I S S CIP S S S S S S I S S S S I I CXM S I R S S S S I R S S S R AMP S R R R R R R R R R R R R AK I S S S I S S I S S S S S CEP S S S S S S S S S S S S S MET S S R R R R R R R R R R R Open in a separate window Cut-off values of different antibiotics according to CLSI guidelines: COT (25 μg): S ≥ 16, I 11–15, R ≤ 10; GEN (10 μg): S ≥ 15, I 13–14, R < 12; CTR (30 μg): S ≥ 21, I 14–20, R ≤ 13; CIP (5 μg): S ≥ 21, I 16–20, R ≤ 15; CXM (30 μg): S ≥ 18, I 15–17, R ≤ 14; AMP (10 μg): S ≥ 29, R ≤ 28; AK (30 μg): S ≥ 17, I 15–16, R ≤ 14; CEP (30 μg): S ≥ 18, I 15–17, R ≤ 14; MET (5 μg): S ≥ 14, I 10–13, R ≤ 9 COT co-trimoxazole, GEN gentamicin, CTR ceftriaxone, CIP ciprofloxacin, CXM cefuroxime, AMP ampicillin, AK amikacin, CEP cephalothin, MET methicillin, S sensitive, I intermediate, R resistant Antibiogram of different strains of S. aureus using methicillin and combi 513 octodisc On the other hand, CFS of all the four Lactobacillus strains ( L. acidophilus , L. casei , L. plantarum , L. paraplantarum ) revealed the anti-staphylococcal activity for the standard, methicillin sensitive and methicillin resistant strains (Table ).

Techniques: Inhibition

Journal: Indian Journal of Microbiology

Article Title: Melioration in Anti-staphylococcal Activity of Conventional Antibiotic(s) by Organic Acids Present in the Cell Free Supernatant of Lactobacillus paraplantarum

doi: 10.1007/s12088-017-0659-z

Figure Lengend Snippet: MIC values and FIC index of antibiotics and CFS alone and in combination against different S. aureus strains as determined by broth dilution technique and checkerboard method

Article Snippet: However, sensitivity of MRSA to other classes of antibiotics such as co-trimoxazole, gentamicin and ciprofloxacin may be due to the inhibition or interference with folate synthesis, protein synthesis (30S) and DNA gyrase, respectively [ 14 – 16 ]. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 ATCC 9144 MSSA S1 MRSA R1 MRSA R2 MRSA R3 MRSA R4 MRSA R5 MRSA R6 MRSA R7 MRSA R8 MRSA R9 MRSA R10 L.paraplantarum COT S S S S I S I I S I S S I GEN S S S S S S S S S S S S S CTR S I R I R I I I I I I S S CIP S S S S S S I S S S S I I CXM S I R S S S S I R S S S R AMP S R R R R R R R R R R R R AK I S S S I S S I S S S S S CEP S S S S S S S S S S S S S MET S S R R R R R R R R R R R Open in a separate window Cut-off values of different antibiotics according to CLSI guidelines: COT (25 μg): S ≥ 16, I 11–15, R ≤ 10; GEN (10 μg): S ≥ 15, I 13–14, R < 12; CTR (30 μg): S ≥ 21, I 14–20, R ≤ 13; CIP (5 μg): S ≥ 21, I 16–20, R ≤ 15; CXM (30 μg): S ≥ 18, I 15–17, R ≤ 14; AMP (10 μg): S ≥ 29, R ≤ 28; AK (30 μg): S ≥ 17, I 15–16, R ≤ 14; CEP (30 μg): S ≥ 18, I 15–17, R ≤ 14; MET (5 μg): S ≥ 14, I 10–13, R ≤ 9 COT co-trimoxazole, GEN gentamicin, CTR ceftriaxone, CIP ciprofloxacin, CXM cefuroxime, AMP ampicillin, AK amikacin, CEP cephalothin, MET methicillin, S sensitive, I intermediate, R resistant Antibiogram of different strains of S. aureus using methicillin and combi 513 octodisc On the other hand, CFS of all the four Lactobacillus strains ( L. acidophilus , L. casei , L. plantarum , L. paraplantarum ) revealed the anti-staphylococcal activity for the standard, methicillin sensitive and methicillin resistant strains (Table ).

Techniques:

Journal: Journal of Clinical and Diagnostic Research : JCDR

Article Title: Use of Triplex PCR for Rapid Detection of PVL and Differentiation of MRSA from Methicillin Resistant Coagulase Negative Staphylococci

doi: 10.7860/JCDR/2013/4523.2731

Figure Lengend Snippet: List of reference strains used for the standardization of the Triplex PCR.

Article Snippet: The method was standardized by using the 10 standard strains of Staphylococci, which included 6 MRSA, 2 S. aureus and 2 CoNS [ ]. table ft1 table-wrap mode="anchored" t5 [Table/Fig-1]: caption a7 S. aureus ATCC 29213 MSSA femA S. aureus ATCC 25923 MSSA femA , pvl S. aureus ATCC 43300 MRSA femA , mecA S. aureus COL MRSA femA , mecA MRSA-Mu50 MRSA femA , mecA MRSA-Mu3 MRSA femA , mecA MRSA-USA300 (FPR3757) MRSA femA , mecA , pvl MRSA-USA400 (MW2) MRSA femA , mecA , pvl S. epidermidis ATCC 12228 MRSE mecA S. epidermidis ATCC 35984 MRSE mecA Open in a separate window List of reference strains used for the standardization of the Triplex PCR.

Techniques:

Journal: Journal of Clinical and Diagnostic Research : JCDR

Article Title: Use of Triplex PCR for Rapid Detection of PVL and Differentiation of MRSA from Methicillin Resistant Coagulase Negative Staphylococci

doi: 10.7860/JCDR/2013/4523.2731

Figure Lengend Snippet: Genes detected among the isolates used for evaluation of the Triplex PCR.

Article Snippet: The method was standardized by using the 10 standard strains of Staphylococci, which included 6 MRSA, 2 S. aureus and 2 CoNS [ ]. table ft1 table-wrap mode="anchored" t5 [Table/Fig-1]: caption a7 S. aureus ATCC 29213 MSSA femA S. aureus ATCC 25923 MSSA femA , pvl S. aureus ATCC 43300 MRSA femA , mecA S. aureus COL MRSA femA , mecA MRSA-Mu50 MRSA femA , mecA MRSA-Mu3 MRSA femA , mecA MRSA-USA300 (FPR3757) MRSA femA , mecA , pvl MRSA-USA400 (MW2) MRSA femA , mecA , pvl S. epidermidis ATCC 12228 MRSE mecA S. epidermidis ATCC 35984 MRSE mecA Open in a separate window List of reference strains used for the standardization of the Triplex PCR.

Techniques: